Development of an LC-MS/MS method for determining 5-MeO-DIPT in dried urine spots and application to forensic cases.
Journal of forensic and legal medicine May 1, 2020 Xiuying Yan, Shuai Yuan, Zhiguo Yu et al.
A dried urine spot (DUS) method using LC-MS/MS was developed to measure the tryptamine hallucinogen 5-MeO-DIPT, which is unstable in liquid urine. Ten microliters of urine were spotted on a card and extracted with methanol. The method's limit of detection was 0.1 ng/ml and lower limit of quantification was 0.2 ng/ml, with accuracy between 98.2% and 103.9% and precision between 2.7% and 8.5%. 5-MeO-DIPT was more stable in DUS than in urine stored at 25 °C. When applied to urine from known users, concentrations ranged from 0.3 to 2.3 ng/ml, lower than those measured by GC-Orbitrap-MS. The small sample volume and simplicity make this useful for drug screening.