Exploring the Frontiers of Psychedelics: A New Chromatographic Method for Detection and Quantification of Psilocybin and Psilocin in Psilocybe cubensis Mushrooms
ACS Omega July 10, 2025 Taynah Pereira Galdino, Lucas C Oliveira, Mateus A Luz et al. 5 citations
A validated HPLC-DAD method quantifies psilocybin and psilocin in psychedelic mushroom extracts for medicinal use. Following Brazilian ANVISA guidelines, the method achieved detection limits of 1.58 mg/L for psilocybin and 1.70 mg/L for psilocin, with quantification limits of 4.78 and 5.17 mg/L, respectively. Recovery intervals ranged from 80–120% for psilocybin and 98–116% for psilocin. The mushroom samples contained 2.57% psilocybin and 0.16% psilocin. Accurate measurement of these compounds supports their reliable use in therapeutic contexts, emphasizing the need for precise quantification in developing safe medical treatments.