Extraction and Characterization of N,N-Dimethyltryptamine from Mimosa tenuiflora: A Multivariate Approach
ACS Omega September 16, 2025 Lucas Cordeiro de Oliveira, Taynah Pereira Galdino, Marcelo Da Silva Pedro et al. 1 citation
N,N-Dimethyltryptamine (DMT), a plant-derived alkaloid, shows therapeutic potential for mental health disorders. An efficient method was established to extract, isolate, and characterize DMT from Mimosa tenuiflora, comparing root bark and stem bark. The stem bark method (sample 2C) yielded 3.45% pure DMT (0.172 g from 5.0003 g powder) and a robust phytochemical profile with alkaloids, tannins, and flavonoids. Characterization confirmed DMT identity via FTIR, HPLC-DAD (retention time 11.81 min), and GC-MS (retention time 16.4 min, 88% spectral similarity). Thermogravimetric analysis showed thermal stability up to 135 °C. Cellular viability exceeded 85% at therapeutic concentrations, with reduction only at 100 μg/mL (53 ± 21%), possibly due to overexposure. Sample 2C is a promising candidate for standardized DMT pharmaceutical formulations.