Analysis of 28 hair samples from users of the hallucinogenic beverage ayahuasca
Forensic Science International April 20, 2021 Mengxi Liu, Huan Yang, Jing Hu et al. 14 citations
A fast and reliable UPLC-MS/MS method was developed and validated for measuring dimethyltryptamine (DMT) in hair samples. The method, which pulverizes 20-milligram hair samples with methanol below 4 °C, achieved a lower limit of quantitation of 3 pg/mg and a calibration curve with R² = 0.992. Intraday and interday precision (RSD < 15%) and accuracy (92-113%) were acceptable, and dilution integrity was confirmed. Applied to 28 forensic cases, DMT concentrations ranged from 3 to 1109 pg/mg.