The Psilocin (4-hydroxy-N,N-dimethyltryptamine) and Bufotenine (5-hydroxy-N,N-dimethyltryptamine) Case: Ensuring the Correct Isomer has Been Identified.
Journal of forensic sciences September 1, 2020 Tehila Mishraki-Berkowitz, Esti Kochelski, Pierce Kavanagh et al. 12 citations
Psilocin and bufotenine are naturally occurring controlled substances, while two other isomers (6-HO-DMT and 7-HO-DMT) are not classified as controlled substances. The four isomers were synthesized and analyzed using thin layer chromatography, Fourier transform infrared spectroscopy, and gas chromatography mass spectroscopy. The methods successfully differentiated all four isomers. Analysis of forensic specimens suspected to be psilocybe mushrooms confirmed that psilocin can be unequivocally identified and its other isomers ruled out, providing accurate identification for forensic investigations.