A highly sensitive UHPLC-MS/MS method for determining 15 designer LSD analogs in biological samples with application to stability studies.
The Analyst January 13, 2025 Olga Wachełko, Karolina Nowak, Kaja Tusiewicz et al. 4 citations
A highly sensitive analytical method using UHPLC-QqQ-MS/MS can detect LSD, 13 of its designer analogs, and a metabolite at femtogram-per-milliliter levels in blood, urine, plasma, and serum. The method separates structural isomers and achieves a limit of quantification of 0.5 pg/mL for all substances. A 30-day stability study showed that collecting samples in sodium fluoride (NaF) tubes stabilizes LSD analogs and minimizes conversion of N1-substituted compounds to LSD or MiPLA. The method is the most sensitive reported for analyzing designer LSD analogs in biological samples and is suitable for routine clinical and forensic use. Fragmentation patterns from mass spectra are proposed to aid future identification of new designer LSD analogs.