Modification of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method targeting lysergic acid diethylamide (LSD) and its primary metabolite (OH-LSD) to include nine LSD analogs.
Journal of forensic sciences September 1, 2024 Amy L Patton, Erin L Karschner, Jeffrey P Walterscheid et al. 1 citation
A validated LC-MS/MS method can detect LSD, its primary metabolite OH-LSD, and nine LSD analogs in urine with a limit of detection of 0.1 ng/mL. The method uses automated sample preparation and a modified analytical column and gradient. Analysis of 325 urine specimens found no LSD analogs, but the procedure is suitable for laboratories expanding their testing scope. Automated preparation reduces manual handling without increasing analytical time. Detection may improve as reference standards for metabolic products become available. The validated procedure supports routine analysis and surveillance of these emerging substances.