A greener, faster method using hollow fiber liquid-phase microextraction (HF-LPME) and LC-MS/MS was developed to measure DMT and three harmala alkaloids in human urine. The method avoids large amounts of toxic solvents. It detects DMT at 1.0 ng/ml and harmala alkaloids at 2.0 ng/ml, with a quantifiable range of 5-200 ng/ml. Precision, accuracy, and recovery (above 80%) met acceptance criteria. Analysis of urine from four subjects confirmed the method's feasibility. The approach offers a simple, time-saving alternative for studying ayahuasca components in biological samples.
A greener analytical method using dispersive liquid-liquid microextraction and LC-MS/MS was developed to measure N,N-dimethyltryptamine (DMT) and three β-carbolines (harmine, harmaline, tetrahydroharmine) in human hair. The method was sensitive, with limits of quantification from 3 to 8 pg/mg, and linear up to 1000 pg/mg. Recovery was low (36-58%), but selectivity showed no interference. Applied to six real samples, DMT concentrations ranged from 21.5 to 204.4 pg/mg, while β-carbolines were generally higher, with harmine reaching over 1000 pg/mg. The method uses less organic solvent than conventional hair extraction, advancing green analytical toxicology for psychoactive alkaloids.