Frontiers in chemistry - Mindscape Collective

Analytical Characterization of 3-MeO-PCP and 3-MMC in Seized Products and Biosamples: The Role of LC-HRAM-Orbitrap-MS and Solid Deposition GC-FTIR.

Frontiers in chemistry January 1, 2020 Giampietro Frison, Flavio Zancanaro, Samuela Frasson et al. 29 citations

Two powders seized by police were identified as the dissociative hallucinogen 3-MeO-PCP and the psychostimulant 3-MMC. Liquid chromatography–high-resolution mass spectrometry (LC-HRAM-Orbitrap-MS) assigned the elemental formulas but could not distinguish between positional isomers of each drug. Solid deposition gas chromatography–Fourier transform infrared spectroscopy (sd-GC-FTIR) unambiguously differentiated the isomers by matching experimental infrared spectra against a library, confirming 3-MeO-PCP and 3-MMC. 3-MeO-PCP was also detected in blood and urine from both subjects, along with dozens of its phase I and phase II metabolites, using LC-HRAM-Orbitrap-MS after deproteinization. sd-GC-FTIR further confirmed 3-MeO-PCP in biological samples. The combined techniques provided full structural characterization without reference standards.

DOI: 10.3389/fchem.2020.618339 Full text 3-methoxyphencyclidine 3-methylmethcathinone Forensic toxicology Gas chromatography +1

Fast Hollow Fiber Liquid-Phase Microextraction as a Greener Alternative for the Determination of N,N-Dimethyltryptamine and Harmala Alkaloids in Human Urine.

Frontiers in chemistry January 1, 2020 Gabriela de Oliveira Silveira, Felipe Rebello Lourenço, Vitor Bruno et al. 12 citations

A greener, faster method using hollow fiber liquid-phase microextraction (HF-LPME) and LC-MS/MS was developed to measure DMT and three harmala alkaloids in human urine. The method avoids large amounts of toxic solvents. It detects DMT at 1.0 ng/ml and harmala alkaloids at 2.0 ng/ml, with a quantifiable range of 5-200 ng/ml. Precision, accuracy, and recovery (above 80%) met acceptance criteria. Analysis of urine from four subjects confirmed the method's feasibility. The approach offers a simple, time-saving alternative for studying ayahuasca components in biological samples.

DOI: 10.3389/fchem.2020.558501 Full text DMT Ayahuasca Lc-ms/ms Green analytical chemistry +2

Papers

Analytical Characterization of 3-MeO-PCP and 3-MMC in Seized Products and Biosamples: The Role of LC-HRAM-Orbitrap-MS and Solid Deposition GC-FTIR.

Fast Hollow Fiber Liquid-Phase Microextraction as a Greener Alternative for the Determination of N,N-Dimethyltryptamine and Harmala Alkaloids in Human Urine.