Psilocybin was isolated from the mushroom Psilocybe argentipes and its presence was determined in specimens of several mushroom species. The work details the chemical isolation and analytical methods used to identify and quantify the alkaloid in these fungi.
Magic mushrooms sold in Japanese markets are difficult to identify by appearance alone, especially when dried and powdered. By analyzing the internal transcribed spacer region of their ribosomal RNA gene, researchers classified the samples into six genetic groups and matched them to known species using DNA databases. Chemical analysis showed that Panaeolus cyanescens contained the highest level of psilocin among the mushrooms tested, while Amanita species contained none.