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Yukiko Makino

Ministry of Health Labour and Welfare

2 papers in the library · 19 citations · publishing 2003-2004

Papers

Discrimination of Psychoactive Fungi (Commonly Called "Magic Mushrooms") Based on the DNA Sequence of the Internal Transcribed Spacer Region.

Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) January 1, 2003 Takuro Maruyama, Osamu Shirota, Nobuo Kawahara et al. 14 citations

Magic mushrooms sold in Japanese markets are difficult to identify by appearance alone, especially when dried and powdered. By analyzing the internal transcribed spacer region of their ribosomal RNA gene, researchers classified the samples into six genetic groups and matched them to known species using DNA databases. Chemical analysis showed that Panaeolus cyanescens contained the highest level of psilocin among the mushrooms tested, while Amanita species contained none.

DOI: 10.3358/shokueishi.44.44 Internal transcribed spacer Sequence biology Genetics

Preparation of Monoclonal Antibodies Reactive to a Hallucinogenic Drug, Psilocin

JOURNAL OF HEALTH SCIENCE January 1, 2004 Mitsune Yamaguchi, Toshiaki Saito, Yoshie Horiguchi et al. 5 citations

A monoclonal antibody (mAb) that reacts to psilocin, a hallucinogenic compound in magic mushrooms, was developed. To create an antigen, the 4-hydroxyl group of psilocin was modified and linked to keyhole limpet hemocyanin. Mice were immunized, and their spleen cells fused with myeloma cells, producing hybridomas that secrete anti-psilocin mAbs. Four clones (BA631, CA231, KA422, MA332) were selected; BA631 and MA332 were IgG1, CA231 and KA422 were IgG2a. Enzyme immunoassay showed BA631 cross-reacts with psilocin and dimethyltryptamine but not with psilocybin, 4-hydroxyindole, tryptamine, or tryptophan. These antibodies may help identify magic mushrooms.

DOI: 10.1248/jhs.50.600 Psilocybin Monoclonal antibody Hallucinogen Keyhole limpet hemocyanin +3