Yoshie Horiguchi

Showa Pharmaceutical University

2 papers in the library · 89 citations · publishing 2004-2014

Papers

Effect of Psilocin on Extracellular Dopamine and Serotonin Levels in the Mesoaccumbens and Mesocortical Pathway in Awake Rats

Biological and Pharmaceutical Bulletin October 23, 2014 Yuichi Sakashita, Kenji Abe, N. Katagiri et al. 84 citations

Psilocin, the active metabolite of psilocybin, increases extracellular dopamine in the nucleus accumbens but not in the ventral tegmental area (VTA) of awake rats. It also raises serotonin (5-HT) levels in the medial prefrontal cortex while decreasing dopamine there. Neither dopamine nor 5-HT levels change in the VTA. Behaviorally, psilocin increases the number of head twitches. The findings suggest psilocin affects the dopaminergic system in the nucleus accumbens and the serotonergic system in the medial prefrontal cortex, acting through the mesoaccumbens and mesocortical pathways.

DOI: 10.1248/bpb.b14-00315 Serotonin Nucleus accumbens Dopaminergic Microdialysis +6

Preparation of Monoclonal Antibodies Reactive to a Hallucinogenic Drug, Psilocin

JOURNAL OF HEALTH SCIENCE January 1, 2004 Mitsune Yamaguchi, Toshiaki Saito, Yoshie Horiguchi et al. 5 citations

A monoclonal antibody (mAb) that reacts to psilocin, a hallucinogenic compound in magic mushrooms, was developed. To create an antigen, the 4-hydroxyl group of psilocin was modified and linked to keyhole limpet hemocyanin. Mice were immunized, and their spleen cells fused with myeloma cells, producing hybridomas that secrete anti-psilocin mAbs. Four clones (BA631, CA231, KA422, MA332) were selected; BA631 and MA332 were IgG1, CA231 and KA422 were IgG2a. Enzyme immunoassay showed BA631 cross-reacts with psilocin and dimethyltryptamine but not with psilocybin, 4-hydroxyindole, tryptamine, or tryptophan. These antibodies may help identify magic mushrooms.

DOI: 10.1248/jhs.50.600 Psilocybin Monoclonal antibody Hallucinogen Keyhole limpet hemocyanin +3