Synthesis of a psilocin hapten and a protein-hapten conjugate
Journal of Pharmacy and Pharmacology September 1, 2002 Christian Albers, Matthias Lehr, Justus Beike et al. 7 citations
Derivatives of psilocin with functionalized alkyl spacers at the indole nitrogen were synthesized as haptens for a radioimmunoassay. The 3-aminopropyl and 4-aminobutyl analogues decomposed during synthesis, but the 3-carboxypropyl derivative was stable. This compound was coupled to bovine serum albumin (BSA) via N-hydroxysuccinimide ester-mediated conjugation. Mass spectrometry showed an average incorporation of 4–5 psilocin hapten molecules per BSA molecule, characterizing the protein–hapten conjugate.