Determination of Buprenorphine, Fentanyl and LSD in Whole Blood by UPLC-MS-MS
Journal of Analytical Toxicology February 18, 2013 Thomas Berg, Benedicte Jørgenrud, Dag Helge Strand 27 citations
A sensitive analytical method using ultra-performance liquid chromatography-tandem mass spectrometry was developed and validated to measure buprenorphine, fentanyl, and lysergic acid diethylamide (LSD) in whole blood. Sample preparation involved liquid-liquid extraction with methyl tert-butyl ether. The method achieved detection limits of 0.28 ng/mL for buprenorphine, 0.044 ng/mL for fentanyl, and 0.0097 ng/mL for LSD. Most phospholipids were removed during extraction, and matrix effects were minimal or absent. Since September 2011, the method has been routinely used at the Norwegian Institute of Public Health for qualitative and quantitative analysis of over 400 whole blood samples, each tested in duplicate.