Detection of metabolites of lysergic acid diethylamide (LSD) in human urine specimens: 2-oxo-3-hydroxy-LSD, a prevalent metabolite of LSD
Journal of Chromatography B Biomedical Sciences and Applications March 1, 1999 Gregory K. Poch, K. L. Klette, Domingo A. Hallare et al. 50 citations
A new LC-MS-MS method for detecting the LSD metabolite 2-oxo-3-hydroxy-LSD (O-H-LSD) in urine is less complex, shorter, and provides cleaner chromatographic characteristics than the existing GC-MS method. All 74 urine specimens containing LSD by GC-MS also contained O-H-LSD at significantly higher concentrations, ranging from 732 to 112,831 pg/ml (mean 16,340 pg/ml). The ratio of O-H-LSD to LSD ranged from 1.1 to 778.1 (mean 42.9). The presence of O-H-LSD at substantially higher concentrations than LSD suggests that analyzing for O-H-LSD as the target analyte using LC-MS-MS will provide a much longer detection window for LSD use than analyzing the parent compound.