Determination of LSD in Urine by Capillary Column Gas Chromatography and Electron Impact Mass Spectrometry
Journal of Analytical Toxicology January 1, 1988 Paula Francom, David M. Andrenyak, Hyun Kyoon Lim et al. 55 citations
A method detects LSD in urine at concentrations as low as 0.5 ng/mL. After adding a deuterium-labeled internal standard, LSD is extracted from urine at pH 8 using n-butyl chloride, then converted to a trimethylsilyl derivative and measured by gas chromatography–mass spectrometry with selected ion monitoring. The procedure tracked LSD concentrations in urine for eight hours after two volunteers each took 70.5 micrograms of LSD orally. Results are compared with those from radioimmunoassay and high-performance liquid chromatography. The report also includes data on LSD stability in urine.