Liquid Chromatography-Electrospray Ionization Mass Spectrometry for the Detection of Lysergide and a Major Metabolite, 2-Oxo-3-Hydroxy-LSD, in Urine and Blood
Journal of Analytical Toxicology October 1, 2000 J. H. Sklerov, Joseph Magluilo, K. K. Shannon et al. 30 citations
A method measures LSD and its metabolite O-H-LSD in urine and blood. O-H-LSD appears in urine at concentrations many times higher than LSD, extending detection time for confirming LSD use. A single-step liquid-liquid extraction on 5-mL urine samples precedes liquid chromatography with electrospray ionization mass spectrometry. Detection limits are 400 pg/mL for O-H-LSD and 100 pg/mL for LSD. In nine LSD-positive urine samples, mean O-H-LSD concentration was 6378 pg/mL (range 332-21371 pg/mL) and mean LSD concentration was 844 pg/mL (range 177-2456 pg/mL), with O-H-LSD levels 0.9 to 19.8 times higher than LSD (mean 10.2). No O-H-LSD was detected in blood samples. Enzymatic hydrolysis of six urine samples showed no significant difference, indicating absence of glucuronic acid conjugation.