The Determination of Lysergide (LSD) in Urine by High-Performance Liquid Chromatography-Isotope Dilution Mass Spectrometry (IDMS)
Journal of Forensic Sciences March 1, 1999 Sa White, A. Kidd, Kenneth S. Webb 23 citations
Isotope dilution mass spectrometry (IDMS) improves the forensic confirmation of lysergic acid diethylamide (LSD) in urine when using liquid chromatography–mass spectrometry (LC-MS). Using a deuterated analog of LSD as an internal standard offers advantages over methysergide. The method achieves a limit of quantification (LOQ) of 0.5 ng/mL, meeting the laboratory's forensic requirement, and can be improved to 0.1 ng/mL under some circumstances. It is linear up to 10 ng/mL LSD in urine and has been validated for accuracy and precision. The study also compares electrospray mass spectra of LSD, LSD-d3, and methysergide, and discusses suitable ions for selected ion monitoring.