Immunoassay Screening of Lysergic Acid Diethylamide (LSD) and its Confirmation by HPLC and Fluorescence Detection Following LSD ImmunElute Extraction
Journal of Analytical Toxicology April 1, 2002 Thomas Grobosch, U. Lemm-Ahlers 13 citations
Of 3872 urine specimens screened for LSD with the CEDIA DAU assay, 48 were initially positive, but only 13 (27%) were confirmed by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) after immunoaffinity extraction. The DPC Coat-a-Count RIA showed complete agreement with HPLC-FLD at concentrations below 500 pg/mL. Fifteen compounds—including ambroxol, diphenhydramine, amitriptyline, and lidocaine—were identified as causing false positives in the CEDIA assay. The IAE/HPLC-FLD method is rapid, reliable, and cost-effective for infrequent LSD analysis, and its chromatographic detection of LSD, nor-LSD, and iso-LSD is unaffected by these cross-reacting compounds even at 100 ng/mL.