Optimization of separation and online sample concentration of N,N‐dimethyltryptamine and related compounds using MEKC
Journal of Separation Science January 9, 2009 M Wang, Chih‐hsin Tsai, Wei‐ya Hsu et al. 11 citations
Optimal separation and online concentration methods for nine tryptamines, including DMT, AMT, and 5-MeO-DMT, were developed using micellar electrokinetic chromatography with UV detection. Limits of detection ranged from 1.0 to 1.8 μg/mL with standard MEKC, improved to 2.2–8.0 ng/mL using sweeping-MEKC, and further to 1.3–2.7 ng/mL using cation-selective exhaustive injection-sweep-MEKC. The migration order of the tryptamines was determined. A spiked human urine sample containing DMT was successfully analyzed, demonstrating practical applicability for drug testing.