Capillary electrophoresis combined with fluorescence spectroscopy, using sodium dodecyl sulfate as a surfactant, separates and concentrates lysergic acid diethylamide (LSD) in mouse blood. Two on-line concentration techniques—sweeping micellar electrokinetic chromatography and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography—were optimized. In a test mouse fed 0.1 mg LSD (about one-tenth the LD50 value for a 20 g mouse), LSD concentrations of 120 ng/mL and 30 ng/mL were detected in blood at 20 and 60 minutes after ingestion, respectively.
Optimal separation and online concentration methods for nine tryptamines, including DMT, AMT, and 5-MeO-DMT, were developed using micellar electrokinetic chromatography with UV detection. Limits of detection ranged from 1.0 to 1.8 μg/mL with standard MEKC, improved to 2.2–8.0 ng/mL using sweeping-MEKC, and further to 1.3–2.7 ng/mL using cation-selective exhaustive injection-sweep-MEKC. The migration order of the tryptamines was determined. A spiked human urine sample containing DMT was successfully analyzed, demonstrating practical applicability for drug testing.