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Electrophoresis

ISSN 1522-2683

3 papers in the library · 171 citations · publishing 1997-2003

Papers

Simultaneous chiral separation of 3,4‐methylenedioxymethamphetamine (MDMA), 3‐4‐methylenedioxyamphetamine (MDA), 3,4‐methylenedioxyethylamphetamine (MDE), ephedrine, amphetamine and methamphetamine by capillary electrophoresis in uncoated and coated capillaries with native β‐cyclodextrin as the chiral selector: Preliminary application to the analysis of urine and hair

Electrophoresis January 1, 1998 Franco Tagliaro, Giulia Manetto, Silvana Bellini et al. 74 citations

A capillary electrophoresis method using native β-cyclodextrin as a chiral selector simultaneously separates and detects the enantiomers of six amphetamine-related substances: ephedrine, amphetamine, methamphetamine, MDMA, MDA, and MDE. Optimized conditions (pH 2.5 phosphate buffer, uncoated capillary, 10 kV) achieve good resolution and chiral selectivity for all analytes. Detection limits are better than 0.2 μg/mL, with high precision (intra-day migration time relative standard deviation < 0.8%) and linearity from 0.156 to 40 μg/mL. After liquid-liquid extraction, urine samples can be analyzed below the 500 ng/mL NIDA cut-off. For hair samples, field-amplified sample stacking enables chiral determination of MDMA, MDA, and MDE at concentrations found in real ecstasy users.

DOI: 10.1002/elps.1150190109 Capillary electrophoresis Chromatography Chemistry Pseudoephedrine +4

Enantioselective determination of 3,4‐methylene‐dioxymethamphetamine and two of its metabolites in human urine by cyclodextrin‐modified capillary zone electrophoresis

Electrophoresis January 1, 1997 Matthias Lanz, Rudolf Brenneisen, Wolfgang Thormann 70 citations

A capillary electrophoresis method using a phosphate buffer with a chiral selector separates the enantiomers of MDMA (Ecstasy) and its metabolites HMMA and MDA in human urine. After enzymatic hydrolysis and solid-phase extraction, detection at 195 nm achieves detection limits of 20–50 ng/mL with 5 mL samples. Analysis of two patients' urine shows enantioselective metabolism: one patient excreted 42.28% of the racemic MDMA dose as R-(−)-MDMA and 10.16% as S-(+)-MDMA; the other excreted 28.63% and 9.34%, respectively. Metabolite enantiomer excretion varied between individuals, demonstrating interindividual differences in MDMA metabolism.

DOI: 10.1002/elps.1150180628 MDMA Chromatography Chemistry Metabolite +6

Determination of lysergic acid diethylamide (LSD) in mouse blood by capillary electrophoresis/ fluorescence spectroscopy with sweeping techniques in micellar electrokinetic chromatography

Electrophoresis March 1, 2003 Ching Fang, Ju‐tsung Liu, S.‐s. Chou et al. 27 citations

Capillary electrophoresis combined with fluorescence spectroscopy, using sodium dodecyl sulfate as a surfactant, separates and concentrates lysergic acid diethylamide (LSD) in mouse blood. Two on-line concentration techniques—sweeping micellar electrokinetic chromatography and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography—were optimized. In a test mouse fed 0.1 mg LSD (about one-tenth the LD50 value for a 20 g mouse), LSD concentrations of 120 ng/mL and 30 ng/mL were detected in blood at 20 and 60 minutes after ingestion, respectively.

DOI: 10.1002/elps.200390119 Lysergic acid diethylamide Micellar electrokinetic chromatography Chemistry Capillary electrophoresis +5