Determination of lysergic acid diethylamide (LSD) in mouse blood by capillary electrophoresis/ fluorescence spectroscopy with sweeping techniques in micellar electrokinetic chromatography
Electrophoresis March 1, 2003 Ching Fang, Ju‐tsung Liu, S.‐s. Chou et al. 27 citations
Capillary electrophoresis combined with fluorescence spectroscopy, using sodium dodecyl sulfate as a surfactant, separates and concentrates lysergic acid diethylamide (LSD) in mouse blood. Two on-line concentration techniques—sweeping micellar electrokinetic chromatography and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography—were optimized. In a test mouse fed 0.1 mg LSD (about one-tenth the LD50 value for a 20 g mouse), LSD concentrations of 120 ng/mL and 30 ng/mL were detected in blood at 20 and 60 minutes after ingestion, respectively.