Analysis of Underivatized Amphetamines and Related Phenethylamines with High-Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry
Journal of Analytical Toxicology March 1, 2000 M Bogusz, Klaus-Dieter Krüger, R. D. Maier 79 citations
A method using liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry (LC-APCI-MS) can separate and identify a wide range of phenethylamines, including amphetamine, methamphetamine, and several designer drugs (MDA, MDEA, MDMA, MBDB, BDMPEA), as well as other related compounds like ephedrine and cathinone, from human serum. The drugs are extracted via solid-phase extraction and then analyzed in a single run. The technique provides clear differentiation between all tested drugs, with detection limits between 1 and 5 micrograms per liter of serum and recoveries ranging from 58 to 96%. The method is suitable for routine forensic toxicology analysis of these substances.