Automated Extraction of Lysergic Acid Diethylamide (LSD) and N-demethyl-LSD from Blood, Serum, Plasma, and Urine Samples Using the Zymark RapidTrace™ with LC/MS/MS Confirmation
Journal of Forensic Sciences May 1, 1998 John de Kanel, W E Vickery, B Waldner et al. 22 citations
A fully automated solid-phase extraction method using the Zymark RapidTrace™ followed by liquid chromatography and tandem mass spectrometry (LC-MS/MS) can quantify lysergic acid diethylamide (LSD) and confirm its metabolite N-demethyl-LSD in blood, serum, plasma, and urine. LSD quantitation uses an internal standard (LSD-d3). The limit of quantitation for LSD is 0.05 ng/mL, and the limit of detection for both LSD and its metabolite is 0.025 ng/mL. Recovery of LSD exceeds 95% at 0.1 and 2.0 ng/mL. At 1.0 ng/mL LSD, within-run relative standard deviation is 2.2% and between-run is 4.4%.