Detection of Lysergic Acid Diethylamide (LSD) in Urine by Gas Chromatography-Ion Trap Tandem Mass Spectrometry
Journal of Analytical Toxicology October 1, 1999 J. H. Sklerov, Kathryn S. Kalasinsky, C. A. Ehorn 19 citations
A confirmatory method using gas chromatography-tandem mass spectrometry (GC-MS-MS) with an internal ionization ion trap detector can detect and quantify lysergic acid diethylamide (LSD) in urine. After a single-step solid-phase extraction of 5 mL of urine, underivatized LSD is measured with limits of quantitation and detection of 80 and 20 pg/mL, respectively. Temperature-programmed on-column injections show linearity over 20-2000 pg/mL (r² = 0.999). Intraday and interday coefficients of variation are below 6% and 13%, respectively. The method has been applied to quality-control specimens and LSD-positive samples, with comparisons to alternative GC-MS methods and extraction procedures.