Determination of LSD in Urine With High-Performance Liquid Chromatography–Mass Spectrometry
Therapeutic Drug Monitoring August 1, 2001 Karl Bodin, Jan‐olof Svensson 11 citations
A method using high-performance liquid chromatography and mass spectrometry can reliably detect LSD in urine at concentrations as low as 0.02 ng/mL. The procedure involves extracting LSD from urine into an organic solvent, then back-extracting into an acetate buffer, followed by separation and detection. A trideuterated form of LSD serves as an internal standard to improve accuracy. The method shows consistent results, with measurement variability of 3.5% within a day and 4.0% between days, and recovers 91% of LSD from samples. The calibration curve is linear across 0.05 to 10 ng/mL, making it suitable for routine testing.