Simultaneous Determination of HFBA-Derivatized Amphetamines and Ketamines in Urine by Gas Chromatography-Mass Spectrometry
Journal of Analytical Toxicology April 1, 2011 H. H. Lee, J. F. Lee, Shin‐yu Lin et al. 35 citations
A single analytical protocol using solid-phase extraction, derivatization with heptafluorobutyric anhydride, and gas chromatography-mass spectrometry can simultaneously measure amphetamine, methamphetamine, MDA, MDMA, MDEA, ketamine, and norketamine in urine. With a 1-mL sample, detection limits range from 15 to 70 ng/mL, and upper limits reach 6000–8000 ng/mL. Precision and accuracy are high, with within-day and between-day coefficients of variation ≤4.95% and accuracy between 96.0% and 110.7% for most analytes. Testing on 107 previously positive urine samples showed good agreement with earlier methods, enabling a single confirmation test for samples screening positive for amphetamines, ketamine, or both.