Bioactive compounds from Psilocybe cubensis mycelial cultures with transient anxiolytic effects in mice.
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society February 1, 2026 K Kala, D Bederska-Lojewska, J Lazur et al.
Chronic microdosing with in vitro cultured Psilocybe cubensis mycelium reduced anxious behavior in male C57BL/6J mice on day 22, but did not affect locomotor activity, depressive, anxiety-related, or anhedonic behaviors at later stages. Psilocybin was present only in the Cambodian strain mycelium at 20.78 mg per 100 g dry weight. The results suggest potential for anxiety prevention, but further studies with higher doses or alternative models are needed to confirm these findings.