Comparison of the separation of nine tryptamine standards based on gas chromatography, high performance liquid chromatography and capillary electrophoresis methods.
Journal of chromatography. A February 15, 2008 Man-Juing Wang, Ju-Tsung Liu, Hung-Ming Chen et al. 12 citations
Nine tryptamines were compared using GC, HPLC, and CE separation techniques. GC/MS and LC/UV-absorption detection limits ranged from 0.5 to 15 µg/mL and 0.3 to 1.0 µg/mL, respectively. CZE/UV-absorption gave detection limits of 0.5–1 µg/mL, while sweeping-MEKC improved detection dramatically to 2–10 ng/mL. Migration times ranged from 11 to 15 minutes by GC, 8 to 23 minutes by HPLC, and 20 to 26 minutes by sweeping-MEKC. The migration order of DMT, DET, DPT, and DBT follows molecular weight, while the order for AMT, 5-MeO-AMT, DIPT, and 5-methoxy-tryptamines can be altered by changing separation conditions.