Journal of chromatography. A
December 18, 2009
Ethan H Mcilhenny, Kelly E Pipkin, Leanna J Standish et al.
58 citations
A new analytical method using direct injection and liquid chromatography-tandem mass spectrometry can simultaneously measure 11 psychoactive compounds in ayahuasca, a plant-based Amazonian beverage used in traditional medicine and religious ceremonies. The technique uses a deuterated internal standard for accurate quantitation and requires only simple dilution of samples up to 200-fold, avoiding complex extraction steps. It shows high specificity, low detection and quantitation limits, and appears to eliminate matrix effects. The method was tested on three different ayahuasca preparations and is expected to aid clinical, ethnobotanical, and forensic studies.
Journal of chromatography. A
February 24, 2012
Brian B Barnes, Nicholas H Snow
25 citations
Salvinorin A, a psychoactive hallucinogen, and related compounds were analyzed in plants, water, and urine using liquid-liquid extraction (LLE), solid-phase microextraction (SPME), and comprehensive two-dimensional gas chromatography-time of flight mass spectrometry (GC×GC-ToFMS). In Salvia divinorum plants, LLE revealed parts-per-billion levels of Salvinorin A and several analogs in leaves and stems, much lower than expected. For water and urine spiked with Salvinorin A, SPME outperformed LLE, achieving a detection limit of about 5 ng/mL, linear range from 8 to 500 ng/mL, and precision around ±10% using external standard quantitation. GC×GC-ToFMS effectively separated target peaks from matrix and chromatographic interferences.
Journal of chromatography. A
July 28, 2017
D Fabregat-Safont, M Barneo-Muñoz, F Martinez-Garcia et al.
24 citations
A new psychoactive substance, 5-methoxy-N-methyl-N-isopropyltryptamine, has emerged after the control of a similar compound, 5-methoxy-N,N-diisopropyltryptamine. This substance was detected in two pill samples from a Spanish smart shop. The metabolism and pharmacokinetics were studied in adult male mice using ultra-high performance liquid chromatography coupled to high resolution mass spectrometry. Four metabolites were identified. The O-demethylated metabolite and the non-metabolised parent compound are proposed as consumption markers in hydrolysed urine. These findings will assist hospitals and forensic laboratories in monitoring consumption and potential intoxication cases related to this tryptamine.
Journal of chromatography. A
February 2, 1996
F R Ley, A R Jeffcoat, B F Thomas
18 citations
A new analytical method using gas chromatography-mass spectrometry with a stable-isotope internal standard can measure ibogaine levels in one milliliter of plasma. The technique is selective and sensitive, addressing a gap in quantitative methods that has limited pharmacokinetic and metabolism studies of ibogaine, a naturally occurring alkaloid being considered as a treatment for drug dependence.
Journal of chromatography. A
August 16, 2024
Nayna Cândida Gomes, Oscar G Cabrices, Bruno Spinosa De Martinis
12 citations
A new method using disposable DPX-SCX extraction tips and gas chromatography-mass spectrometry can simultaneously detect and quantify 16 psychoactive substances—including amphetamines, cocaine, fentanyl, and several novel psychoactive substances—in sweat samples collected with laboratory-developed patches. Validation showed quantification limits between 2 and 30 ng per patch and linearity from 2 to 1100 ng per patch. When applied to 30 sweat samples from volunteer drug users, the method detected cocaine, MDMA, nicotine, caffeine, and other compounds, with recovery rates of 72.4% to 97.1%. Sweat is a viable, non-invasive biological matrix for monitoring drug use, particularly in rehabilitation settings.
Journal of chromatography. A
February 15, 2008
Man-Juing Wang, Ju-Tsung Liu, Hung-Ming Chen et al.
12 citations
Nine tryptamines were compared using GC, HPLC, and CE separation techniques. GC/MS and LC/UV-absorption detection limits ranged from 0.5 to 15 µg/mL and 0.3 to 1.0 µg/mL, respectively. CZE/UV-absorption gave detection limits of 0.5–1 µg/mL, while sweeping-MEKC improved detection dramatically to 2–10 ng/mL. Migration times ranged from 11 to 15 minutes by GC, 8 to 23 minutes by HPLC, and 20 to 26 minutes by sweeping-MEKC. The migration order of DMT, DET, DPT, and DBT follows molecular weight, while the order for AMT, 5-MeO-AMT, DIPT, and 5-methoxy-tryptamines can be altered by changing separation conditions.
Journal of chromatography. A
August 14, 2009
Cláudia P.B. Martins, Sally Freeman, John F. Alder et al.
11 citations
A purported two-step internet recipe for making the psychoactive compound DMT was tested and found to produce almost none. The second step, methylation of tryptamine, was analyzed with advanced mass spectrometry. Instead of DMT, the reaction yielded 47.4% 1-N-methyl-TMT, 21.0% TMT, 11.1% unreacted tryptamine, and a 0.5% trace of N-methyltryptamine. The work demonstrates that synthetic methods circulated online can be unreliable and produce largely unintended, non-psychoactive products.
Journal of chromatography. A
October 11, 2023
Christiane Martins de Vasconcellos Silveira, Vanessa Farelo Dos Santos, Isis Moraes Ornelas et al.
3 citations
Lysergic Acid Diethylamide (LSD) is metabolized in the model organism Caenorhabditis elegans, producing a range of metabolites identified through ultra-high performance liquid chromatography coupled with high-resolution tandem mass spectrometry. The findings illuminate metabolic pathways and pharmacokinetics of LSD, demonstrating that C. elegans is a suitable system for studying psychedelic compound metabolism and supporting further research into therapeutic applications.
Journal of chromatography. A
July 19, 2025
Sabrina Islam, Tiffany Liden, Roman Goff et al.
1 citation
Psilocybin mushrooms, which contain psychoactive compounds like psilocybin and psilocin, are being studied for treating psychological disorders. An untargeted chemical analysis using liquid chromatography and mass spectrometry compared the chemical diversity of several psilocybin mushroom species to edible non-psilocybin mushrooms. The analysis identified multiple classes of compounds and their derivatives. Principal component analysis showed that psilocybin mushrooms and non-psilocybin mushrooms are compositionally very different from each other. These findings deepen understanding of the chemical complexity of psilocybin mushrooms and provide a foundation for future research into their medical and psychological applications.