Analysis of Salvinorin A in plants, water, and urine using solid-phase microextraction-comprehensive two-dimensional gas chromatography-time of flight mass spectrometry.
Journal of chromatography. A February 24, 2012 Brian B Barnes, Nicholas H Snow 25 citations
Salvinorin A, a psychoactive hallucinogen, and related compounds were analyzed in plants, water, and urine using liquid-liquid extraction (LLE), solid-phase microextraction (SPME), and comprehensive two-dimensional gas chromatography-time of flight mass spectrometry (GC×GC-ToFMS). In Salvia divinorum plants, LLE revealed parts-per-billion levels of Salvinorin A and several analogs in leaves and stems, much lower than expected. For water and urine spiked with Salvinorin A, SPME outperformed LLE, achieving a detection limit of about 5 ng/mL, linear range from 8 to 500 ng/mL, and precision around ±10% using external standard quantitation. GC×GC-ToFMS effectively separated target peaks from matrix and chromatographic interferences.