Ecotoxicology and Environmental Safety
January 31, 2024
Sen Zhao, Jinyuan Chen, Chenhao Zhong et al.
3 citations
5-MeO-MiPT, a novel psychoactive tryptamine, caused inhibited movement and anxiety-like behavior in adult zebrafish. Metabolomic analysis after drug injection revealed 22 distinct potential biomarkers and seven significantly altered metabolic pathways, including amino acid, lipid, and energy metabolism. The metabolic changes indicated observable brain damage, especially disruption in the liver-brain pathway, linking the drug's metabolic effects to the anxiety-like behavior.
Ecotoxicology and Environmental Safety
August 30, 2024
Sen Zhao, Meng Liu, Jinyuan Chen et al.
1 citation
5-Methoxy-N-isopropyl-N-methyltryptamine (5-MeO-MiPT), a tryptamine-derived novel psychoactive substance detected in aquatic environments, alters gene expression and disrupts signaling pathways in zebrafish. After 30 days of injection with varying concentrations, RNA-seq, qPCR, metabolomics, and histopathology showed that 5-MeO-MiPT significantly affects the transcription of 13 genes, including ucp1, pet100, grik3, and grik4, via the Gα q/11-PLCβ signaling pathway. The substance inhibits DAG-Ca2+/Pkc/Erk, Pkc/Pla2/PLCs, and Ca2+/CamkII/NMDA pathways while enhancing Ca2+/Creb, mechanisms that may mediate behavioral inhibition and oxidative stress. These findings clarify toxicological and addiction mechanisms of 5-MeO-MiPT and suggest approaches for studying other tryptamine-based NPS and diagnosing liver-brain pathway diseases.
Journal of Applied Toxicology
April 8, 2026
Yin Tang, Yanjiao Wang, Liang Meng et al.
Exposure to the hallucinogenic drug 5-MeO-DiPT for a short period altered 27 metabolites in the brains of zebrafish. Eight metabolites increased and 19 decreased, while nine core metabolic pathways were significantly disrupted. The drug disturbed neurotransmitter balance, amino acid metabolism, and lipid peroxidation, leading to impairments in neural conduction, immune response, and energy metabolism. The findings suggest potential carcinogenic risks and a mechanism for inducing metabolic syndrome.