How to sample a seizure plant: the role of the visualization spatial distribution analysis of Lophophora williamsii as an example.
Forensic sciences research June 1, 2023 Jiaman Lin, Keming Yun, Qiran Sun et al. 2 citations
Mescaline is concentrated in the epidermal tissues and the meristematic tissues of the crown of Lophophora williamsii (peyote), as shown by a validated MALDI mass spectrometry imaging method. Low-temperature storage at -80°C, drying of flower samples, and cutting 40 μm thick sections at -20°C using gelatin as embedding medium are appropriate preparation conditions. Using DCTB as an auxiliary matrix and a laser intensity of 45 are favorable parameters for mescaline analysis. These findings provide a basis for determining the best sampling locations for mescaline in peyote and offer a reference for optimizing storage and preparation conditions for raw plant organs before MALDI detection.