Bioanalysis
March 1, 2010
Graham Lappin
36 citations
Microdosing, a technique used for about ten years, allows researchers to compare the pharmacokinetics of a very low drug dose with those at a therapeutic dose. About 80% of available microdose pharmacokinetic data scale within a twofold difference of therapeutic doses. The method is expanding beyond pharmacokinetic prediction into areas like studying drug-drug interactions, where volunteers receive a microdose before and after an enzyme-inhibiting or -inducing drug. It also helps obtain early metabolic profiles by administering a 14C-labeled drug and comparing total and unchanged compound concentrations. Microdosing is now being applied to assess drug concentrations in key cell or tissue types, broadening its use in drug development.
Bioanalysis
July 1, 2012
Carolina Dizioli Rodrigues de Oliveira, Guilherme Gonçalves Okai, José Luiz Costa et al.
33 citations
A simple and rapid method was developed to estimate administered doses of ayahuasca, supporting further pharmacological and toxicological investigations of ayahuasca exposure.
Bioanalysis
March 27, 2009
Hong-Wu Shen, Xi-Ling Jiang, Ai‐ming Yu
26 citations
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously measure the psychedelic drug 5-MeO-DMT and its active metabolite bufotenine in mouse serum. Using a simple protein precipitation and 9-minute run, the assay was linear over a wide concentration range with high precision and accuracy. After injecting mice with 2 mg/kg of 5-MeO-DMT, the systemic exposure to bufotenine was about 1/14 that of the parent drug. Because bufotenine binds the 5-HT2A receptor with roughly ten times higher affinity than 5-MeO-DMT, the metabolite may substantially contribute to the drug's overall pharmacological and toxic effects.
Bioanalysis
July 1, 2016
Cláudia Margalho, Francisco Corte-Real, Manuel López-rivadulla et al.
The main psychoactive component of Salvia divinorum, salvinorin A, can be identified and quantified in biological specimens, which is important for forensic and clinical toxicology. Despite research efforts, uncontrolled abuse of the substance has increased, posing serious short- and long-term health risks. Alternative biological matrices, such as those used as complementary postmortem samples or when blood and urine are unavailable, can provide useful information about prior exposure. This article summarizes legal aspects of Salvia divinorum and salvinorin A, along with analytical methods for determining salvinorin A in biological matrices.