Determination of lysergic acid diethylamide (LSD) in mouse blood by capillary electrophoresis/ fluorescence spectroscopy with sweeping techniques in micellar electrokinetic chromatography
Ching Fang, Ju‐tsung Liu, S.‐s. Chou, Cheng‐huang Lin
Electrophoresis March 1, 2003 DOI: 10.1002/elps.200390119 via OpenAlex
Summary
AI-generated from the abstractCapillary electrophoresis combined with fluorescence spectroscopy, using sodium dodecyl sulfate as a surfactant, separates and concentrates lysergic acid diethylamide (LSD) in mouse blood. Two on-line concentration techniques—sweeping micellar electrokinetic chromatography and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography—were optimized. In a test mouse fed 0.1 mg LSD (about one-tenth the LD50 value for a 20 g mouse), LSD concentrations of 120 ng/mL and 30 ng/mL were detected in blood at 20 and 60 minutes after ingestion, respectively.
Study at a glance
| Characteristics | Experimental study Peer reviewed |
|---|---|
| Sample size | 1 |
| Population | Mouse blood |
| Intervention | LSD |
| Dose | 0.1 mg |
| Duration | 60 minutes |
| Topics | LSD |
| Keywords | Micellar electrokinetic chromatography Chemistry Capillary electrophoresis Sodium dodecyl sulfate Electrokinetic phenomena |
| Citations | 27 |
| Key finding | LSD concentrations of 120 ng/mL and 30 ng/mL were detected in mouse blood at 20 and 60 minutes after ingestion, respectively. |
Abstract
Abstract The separation and on‐line concentration of lysergic acid diethylamide (LSD) in mouse blood was achieved by means of capillary electrophoresis/fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as the surfactant. Techniques involving on‐line sample concentration, including sweeping micellar electrokinetic chromatography (sweeping‐MEKC) and cation‐selective exhaustive injection‐sweep‐micellar electrokinetic chromatography (CSEI‐sweep‐MEKC) were applied; the optimum on‐line concentration and separation conditions were determined. In the analysis of an actual sample, LSD was found in a blood sample from a test mouse (0.1 mg LSD fed to a 20 g mouse; ∼1/10 to the value of LD 50 ). As a result, 120 and 30 ng/mL of LSD was detected at 20 and 60 min, respectively, after ingestion of the doses.