Optimization of the separation of lysergic acid diethylamide in urine by a sweeping technique using micellar electrokinetic chromatography
Journal of Chromatography B Analytical Technologies in the Biomedical and Life Sciences July 25, 2002 DOI: 10.1016/s1570-0232(02)00268-4 via Elsevier
Summary
AI-generated from the abstractA method using capillary electrophoresis with fluorescence detection was developed to separate and concentrate LSD and related compounds (iso-LSD and LAMPA) in human urine. The technique employed the surfactant SDS and optimized buffer pH, surfactant concentration, and organic solvent content. Two on-line concentration approaches, sweeping-MEKC and CSEI-sweep-MEKC, were evaluated for their sensitivity, precision, and simplicity. The work describes the analytical procedure and compares the performance of these concentration techniques for detecting these compounds in urine samples.
Study at a glance
| Characteristics | Method development and validation Peer reviewed |
|---|---|
| Population | Human urine samples |
| Citations | 24 |
| Key finding | The sweeping-MEKC and CSEI-sweep-MEKC techniques were successfully applied for the on-line concentration and separation of LSD, iso-LSD, and LAMPA in human urine, with the advantages and disadvantages of each approach discussed. |
Abstract
The separation and on-line concentrations of lysergic acid diethylamide (LSD), iso-lysergic acid diethylamide (iso-LSD) and lysergic acid N,N-methylpropylamide (LAMPA) in human urine were investigated by capillary electrophoresis-fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as an anionic surfactant. A number of parameters such as buffer pH, SDS concentration, Brij-30 concentration and the content of organic solvent used in separation, were optimized. The techniques of sweeping-micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were used for determining on-line concentrations. The advantages and disadvantages of this procedure with respect to sensitivity, precision and simplicity are discussed and compared.