A validated method detects 32 synthetic stimulant and hallucinogenic drugs, often sold as bath salts, in oral fluid. Using simple protein precipitation and a 7.5-minute chromatographic separation, the technique achieves detection limits of 1 ng/mL and quantitation from 2.5 to 500 ng/mL with high accuracy (85.3-108.4%) and precision (1.9-14%). Applied to 12 routine samples, two contained 2-CB and DOB (5 and 4 ng/mL) and MPBP and TFMPP (both 4 ng/mL). The method enables rapid screening of these compounds in oral fluid, applicable to routine testing laboratories.
Classic psychedelics like psilocybin, ketamine, and LSD activate 5HT2A serotonin receptors and produce individualized subjective effects. Public interest in psychedelic medicine is high, but clinical evidence remains limited. Psilocybin and psilocin are tryptophan-based alkaloids found in several mushroom genera. This review covers the current evidence for psilocybin as a clinical medicine, its chemistry, proposed therapeutic mechanism, and future research directions for psilocybin-based therapies.