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Sanah Malomile Nkadimeng

University of Pretoria

5 papers in the library · 176 citations · publishing 2020-2023

Papers

Anti-Inflammatory Effects of Four Psilocybin-Containing Magic Mushroom Water Extracts in vitro on 15-Lipoxygenase Activity and on Lipopolysaccharide-Induced Cyclooxygenase-2 and Inflammatory Cytokines in Human U937 Macrophage Cells

Journal of Inflammation Research August 1, 2021 Sanah Malomile Nkadimeng, Christiaan Ml Steinmann, J.n. Eloff 77 citations

Hot-water extracts from four types of psilocybin-containing magic mushrooms may reduce inflammation by decreasing levels of pro-inflammatory signaling molecules. The findings suggest these extracts have potential anti-inflammatory properties through the suppression of inflammatory mediators.

Phytochemical, Cytotoxicity, Antioxidant and Anti-Inflammatory Effects of Psilocybe Natalensis Magic Mushroom

Plants August 31, 2020 Sanah Malomile Nkadimeng, Alice Nabatanzi, Christiaan M.l. Steinmann et al. 66 citations

Extracts from Psilocybe natalensis, a psilocybin-containing mushroom from South Africa, show anti-inflammatory and antioxidant effects in lab tests on mouse immune cells. Boiling hot water, cold water, and ethanol extracts all reduced nitric oxide, prostaglandin E2, and interleukin 1β production in lipopolysaccharide-stimulated macrophages in a dose-dependent manner, similar to positive controls quercetin and N-nitro-L-arginine methyl ester. The extracts were safe at the concentrations used. Phytochemical analysis confirmed the presence of natural antioxidant and anti-inflammatory compounds.

Effects and safety of Psilocybe cubensis and Panaeolus cyanescens magic mushroom extracts on endothelin-1-induced hypertrophy and cell injury in cardiomyocytes

Scientific Reports December 18, 2020 Sanah Malomile Nkadimeng, Christiaan M.l. Steinmann, J.n. Eloff 31 citations

The prevalence of major depression is higher in people with chronic heart failure than in the general population. Psilocybin-containing mushrooms have been used historically for their mind-healing properties, but their safety in cardiovascular disease is not fully known. This study investigated the effects of water extracts from Psilocybe cubensis and Panaeolus cyanescens on endothelin-1-induced pathological hypertrophy and TNF-α-induced cell injury in H9C2 cardiomyocytes. The extracts did not aggravate hypertrophy and protected against TNF-α-induced injury and cell death at the concentrations used. The results support medicinal safe use under controlled conditions and caution against higher concentrations.

Administration effects of four psilocybin mushroom extracts on serotonin levels and endothelial nitric oxide synthase activity levels in vivo and in vitro after one hour

Research Square (Research Square) July 18, 2023 Sanah Malomile Nkadimeng, L. Hay, Christiaan Ml Steinmann et al. 1 citation

Four species of psilocybin-containing mushrooms—Panaeolus cyanescens, Psilocybe natalensis, Psilocybe cubensis, and a leucistic A+ strain of Psilocybe cubensis—were tested in anesthetized Wistar rats for their effects on heart function, serotonin, and nitric oxide (NO) pathways. Hot-water extracts of the mushrooms (5 mg/kg) caused a non-significant increase in left ventricular (LV) parameters after 20 minutes, unlike the immediate effect of the control drug LNAME. The mushrooms significantly raised serotonin levels and suppressed endothelial nitric oxide synthase (eNOS) activity both in vivo and in vitro on H9C2 cardiomyocytes. The findings suggest that disruption of eNOS pathways may underlie temporary blood pressure increases from psilocybin mushrooms, with different species producing distinct cardiac effects.

Cardioprotective Potential of the Ethanol and Water Extracts of Four Psilocybin Mushrooms on Angiotensin II-Induced Hypertrophy and Oxidative Stress on H9C2 Cardiomyocytes

Preprints.org June 6, 2023 Sanah Malomile Nkadimeng, Christiaan M.l. Steinmann, J.n. Eloff 1 citation preprint

Extracts from four psilocybin-containing mushrooms—Panaeolus cyanescens, Psilocybe natalensis, Psilocybe cubensis, and Psilocybe cubensis leucistic A+ strain—did not worsen angiotensin II-induced cardiac hypertrophy in rat heart cells and instead showed protective effects against oxidative stress. Angiotensin II reduced cell viability, increased cell width, and raised reactive oxygen species levels. The mushroom extracts, prepared with ethanol, cold water, or hot water, did not exacerbate these changes; they exhibited cardio-protective activity. Losartan, an angiotensin receptor blocker, served as a positive control. Phytochemical analysis detected known antioxidant and anti-inflammatory compounds in the extracts.