Psilocybin, a naturally occurring psychedelic alkaloid, was measured in dried fruiting-bodies of the mushroom Inocybe aeruginascens using high-performance liquid chromatography. Its concentration was lower than that found in two Psilocybe species, Psilocybe bohemica and Psilocybe semilanceata. As with Psilocybe species, the psilocybin content in Inocybe decreased during storage of the dried mushrooms. The amount also varied depending on where the fruiting-bodies were collected.
Thin-layer chromatography confirmed psilocybin in Psilocybe semilanceata and P. coprinifacies collected in Czechoslovakia. For P. semilanceata, this is the first proof of psilocybin in native fruit bodies; for P. coprinifacies, it is a new finding. The concentration of psychotropic compounds was species-specific and stable. Psilocin was not definitively detected.