LC-MS Analysis of Human Urine Specimens for 2-Oxo-3-Hydroxy LSD: Method Validation for Potential Interferants and Stability Study of 2-Oxo-3-Hydroxy LSD Under Various Storage Conditions
Journal of Analytical Toxicology May 1, 2002 K. L. Klette, C. K. Horn, P. R. Stout et al. 39 citations
A major metabolite of LSD, 2-oxo-3-hydroxy lysergic acid diethylamide (O-H-LSD), is a superior marker for identifying LSD use because it appears in urine at concentrations 16 to 43 times higher than LSD itself. Testing a wide range of chemically similar compounds, over-the-counter products, prescription drugs, and other drugs of abuse showed none interfered with detecting O-H-LSD. The metabolite remained stable under refrigerated and frozen conditions within normal urine pH (4.6-8.4), but significant loss occurred at room temperature or higher (24-50°C). These findings support the forensic reliability of liquid chromatography-mass spectrometry for detecting LSD use via O-H-LSD.