Development and Validation of a Nonisotopic Immunoassay for the Detection of LSD in Human Urine
Journal of Analytical Toxicology October 1, 1996 N. P. Cassells, Derek H. Craston, C. W. Hand et al. 15 citations
A new microplate enzyme immunoassay (EIA) detects lysergic acid diethylamide (LSD) in human urine by having LSD in the sample compete with immobilized LSD for binding to rabbit anti-LSD antibodies. The assay requires only 25 microliters of urine and three incubation periods at room temperature. It showed no cross-reaction with other drugs, including ergot alkaloids at up to 100,000 ng/mL, and only slight cross-reactivity with ergonovine (0.0008%). Nor-LSD cross-reacted between 16% and 28%. At a qualitative cutoff of 0.5 ng/mL, precision testing gave a coefficient of variation of 6%.