The Analysis of Lysergide (LSD): The Development of Novel Enzyme Immunoassay and Immunoaffinity Extraction Procedures Together with an HPLC-MS Confirmation Procedure
Journal of Forensic Sciences November 1, 1996 Kenneth S. Webb, Pb Baker, N. P. Cassells et al. 42 citations
A forensic procedure for detecting lysergide (LSD) in urine uses a novel enzyme immunoassay (EIA) and immunoaffinity extraction alongside an established radioimmunoassay (RIA). Initial screening is followed by quantitative estimation via high-performance liquid chromatography with fluorescence detection after solid phase extraction. Final confirmation and quantitation, without derivatization, uses HPLC with electrospray ionization mass spectrometry and methysergide as an internal standard. The detection limit is 0.5 ng/mL. A blind trial confirmed the results. The study discusses internal standard choice, LSD's photo-sensitivity, and shows no interferants among a wide range of compounds tested. Comparisons are made between extraction and screening methods.