Use of Coconut Charcoal and Menthone-Thiosemicarbazone Polymer as Solid Phase Materials for the Determination of N,N-Dimethyltryptamine, Harmine, Harmaline, Harmalol, and Tetrahydroharmine in Ayahuasca Beverage by Liquid Chromatography-Tandem Mass Spectrometry
Journal of the Brazilian Chemical Society January 1, 2018 Sandro Navickiene, Luı́s M. N. B. F. Santos, Mônica C. Santos et al. 2 citations
A solid-phase extraction method using ultra-performance liquid chromatography-tandem mass spectrometry was developed to quantify five major alkaloids—N,N-dimethyltryptamine, harmine, harmaline, harmalol, and tetrahydroharmine—in ayahuasca. Two new adsorbent materials, coconut charcoal and menthone-thiosemicarbazone polymer, were tested. Recoveries ranged from 40.6% to 116.2%, with detection limits between 0.003 and 0.016 μg mL⁻¹ and quantification limits between 0.009 and 0.043 μg mL⁻¹. The method was linear over 0.05–1.0 μg mL⁻¹ (correlation coefficients 0.9969–0.9998). Applied to an ayahuasca sample from a religious group in Fortaleza, Brazil, alkaloid concentrations ranged from 0.3 to 19.3 mg mL⁻¹.