Modeling Retention Behavior on Analysis of Hallucinogenic Mushrooms Using Hydrophilic Interaction Liquid Chromatography
Journal of Chromatographic Science November 15, 2018 Norbert Rácz, Júlia Nagy, Wen Jiang et al. 12 citations
Three hydrophilic interaction liquid chromatography (HILIC) charge modulated amide columns—iHILIC®-Fusion, iHILIC®-Fusion(+), and iHILIC®-Fusion(P)—were compared for separating hallucinogenic alkaloids (psilocin, psilocybin, and baeocystin) from an extract of a truffle-like fungus. Three modeling methods predicted retention times in isocratic separation as a function of mobile phase composition, pH, and temperature. All columns separated the two hallucinogenic alkaloids from each other and from matrix components, with most compounds achieving satisfactory resolution. The quadratic modeling approach best predicted chromatograms under predefined conditions, the exponential model performed worst, and multivariate data analysis fell between the two.