Biochemical pharmacology
May 1, 1987
B R Sitaram, L Lockett, R Talomsin et al.
75 citations
After injection into the abdominal cavity, 5-methoxy-N,N-dimethyltryptamine and N,N-dimethyltryptamine are rapidly taken up by and cleared from all tissues examined. Live-animal experiments confirm earlier lab findings that these compounds are metabolized through oxidative deamination, N-demethylation, O-demethylation, and N-oxidation. Analysis of metabolic profiles across tissues identified N-oxides as major metabolites. Pretreating animals with iproniazid successfully inhibited and redirected metabolism away from indole acids toward the parent compounds and their structurally unique metabolites.
Biological psychiatry
November 15, 1990
B R Sitaram, W R Mcleod
29 citations
The psychotomimetic indolealkylamines N,N-dimethyltryptamine, 5-methoxy-N,N-dimethyltryptamine, and 5-hydroxy-N,N-dimethyltryptamine have been found in human body fluids, but their link to psychotic illness is still debated. Studies in rats show these compounds are rapidly metabolized and excreted by the kidneys. This rapid clearance may explain inconsistencies in past clinical research and should inform the design of future studies.
Biochemical pharmacology
May 1, 1987
B R Sitaram, R Talomsin, G L Blackman et al.
23 citations
Using liquid chromatography techniques, metabolites of the psychotomimetic compounds N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine were separated and characterized after incubation with rat tissue extracts. In liver, kidney, and brain extracts, metabolic routes included oxidative deamination, N-demethylation, O-demethylation, and N-oxidation. The quantitative importance of each route was assessed using N,N-dimethyltryptamine as a substrate.
Journal of chromatography
November 27, 1987
B R Sitaram, L Lockett, M Mcleish et al.
9 citations
A new method combining liquid chromatography with gas chromatography-mass spectrometry provides unequivocal verification of structurally characteristic metabolites of the psychotomimetic indolealkylamines N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine. Previously, metabolite identity was confirmed using multiple analytical techniques including multidimensional liquid chromatography and stop-flow spectroscopic analysis. The described approach enables separation, characterization, and quantitation of metabolites formed both in vitro and in vivo.