Preparation of Monoclonal Antibodies Reactive to a Hallucinogenic Drug, Psilocin
JOURNAL OF HEALTH SCIENCE January 1, 2004 Mitsune Yamaguchi, Toshiaki Saito, Yoshie Horiguchi et al. 5 citations
A monoclonal antibody (mAb) that reacts to psilocin, a hallucinogenic compound in magic mushrooms, was developed. To create an antigen, the 4-hydroxyl group of psilocin was modified and linked to keyhole limpet hemocyanin. Mice were immunized, and their spleen cells fused with myeloma cells, producing hybridomas that secrete anti-psilocin mAbs. Four clones (BA631, CA231, KA422, MA332) were selected; BA631 and MA332 were IgG1, CA231 and KA422 were IgG2a. Enzyme immunoassay showed BA631 cross-reacts with psilocin and dimethyltryptamine but not with psilocybin, 4-hydroxyindole, tryptamine, or tryptophan. These antibodies may help identify magic mushrooms.