Progress in Neuro-Psychopharmacology and Biological Psychiatry
January 10, 2012
Evan J. Kyzar, Christopher Collins, Siddharth Gaikwad et al.
122 citations
Mescaline and phencyclidine (PCP) dose-dependently increased top activity in the novel tank test, reduced immobility, and disrupted swimming patterns in zebrafish. PCP, but not mescaline, evoked circling behavior in the open field test. At the highest doses tested, mescaline markedly increased shoaling behavior, while PCP did not affect it. Whole-body cortisol levels were unchanged by 20 mg/l mescaline but elevated by 3 mg/l PCP. These findings indicate that zebrafish models are sensitive to hallucinogenic compounds, producing complex behavioral and physiological effects.
Behavioural Pharmacology
April 8, 2011
Adam Stewart, Russell Riehl, Keith Wong et al.
66 citations
Acute exposure to high doses of MDMA (40-120 mg/l) reduces bottom swimming and immobility in zebrafish and impairs intrasession habituation at doses as low as 10 mg/l, while lower doses (0.25-10 mg/l) show no behavioral effects. MDMA also increases brain c-fos expression. These findings support the use of zebrafish as a model for screening hallucinogenic compounds.
Open MIND
January 1, 2015
Jonathan Cachat, Chris Collins, Evan J. Kyzar et al.
Three-dimensional reconstructions of zebrafish swimming paths enable both macro- and micro-level analysis of behavior, offering a more complete picture than traditional 2D traces. Temporal 3D reconstructions plot spatial data across time to reflect activity over testing, while spatial 3D reconstructions use two cameras to depict activity within the actual arena. These reconstructions are highly sensitive to anxiolytic, anxiogenic, and hallucinogenic effects in adult zebrafish. For example, ibogaine reversed natural behaviors, a characterization impossible without 3D reconstructions. Track3D, applied for the first time in adult zebrafish, showed strong significant correlation (R>0.07) of automated endpoints with manual data, providing precise calculation of movement parameters and accurate spatiotemporal integration. These approaches permit advanced movement pattern analysis for screening psychoactive compounds.