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Biomedicines

ISSN 2227-9059

15 papers in the library · 368 citations · publishing 2020-2026

Papers

Psilocybin as a New Approach to Treat Depression and Anxiety in the Context of Life-Threatening Diseases—A Systematic Review and Meta-Analysis of Clinical Trials

Biomedicines September 5, 2020 Ana Sofia Vargas, Ângelo Luís, Mário Barroso et al. 121 citations

A systematic review with meta-analysis of clinical trials found that psilocybin significantly reduces symptoms of depression and anxiety in patients with life-threatening diseases. Analyzing data from 92 patients, the intervention group showed a significant decrease in depression scores on the Beck Depression Inventory and in both trait and state anxiety scores on the State-Trait Anxiety Inventory. The results suggest psilocybin may be effective for conditions resistant to standard treatments or where pharmacotherapy is not yet approved, and it may also have potential as a first-line treatment given its safety profile.

Neurobiological Changes Induced by Mindfulness and Meditation: A Systematic Review.

Biomedicines November 15, 2024 83 citations

Mindfulness and meditation practices, particularly Mindfulness-Based Stress Reduction (MBSR), improve emotional regulation and brain structure, reduce anxiety, and enhance stress resilience. These practices induce neuroplasticity, increase cortical thickness, reduce amygdala reactivity, and improve brain connectivity and neurotransmitter levels. MBSR enhances brain regions involved in emotional processing and sensory perception, improves psychological outcomes such as anxiety and depression, and exhibits unique mechanisms of pain reduction compared to placebo. Future research should focus on diverse populations and naturalistic settings to better understand and optimize these benefits.

Ketamine, Esketamine, and Arketamine: Their Mechanisms of Action and Applications in the Treatment of Depression and Alleviation of Depressive Symptoms.

Biomedicines October 9, 2024 Piotr Kawczak, Igor Feszak, Tomasz Bączek 50 citations

Esketamine, the (S)-enantiomer of ketamine, is more potent than arketamine, offering three times stronger analgesic effects and 1.5 times greater anesthetic efficacy. It provides smoother anesthesia with fewer side effects and is used clinically for its neuroprotective, bronchodilatory, and antiepileptic properties. Approved by the FDA and EMA in 2019, esketamine is prescribed alongside SSRIs or SNRIs for treatment-resistant depression. Arketamine, the (R)-enantiomer, shows potential for treating neurological disorders like Alzheimer's, Parkinson's, and multiple sclerosis, with possible antidepressant and anti-inflammatory benefits. Esketamine is already in clinical use, while arketamine requires further research on safety, efficacy, and dosing.

Indole Alkaloids from Psychoactive Mushrooms: Chemical and Pharmacological Potential as Psychotherapeutic Agents

Biomedicines February 5, 2023 Erika Plazas, Nicoletta Faraone 40 citations

Indole alkaloids from magic mushrooms are promising alternatives to synthetic drugs for treating neuropsychiatric disorders like depression, anxiety, and PTSD. These compounds have a rich history and broad biological properties, with the indole heterocycle being significant in drug discovery. The review reports the physicochemical and pharmacological characteristics of these alkaloids, highlighting their potential as safe and effective therapeutic agents.

Rostral Anterior Cingulate Thickness Predicts the Emotional Psilocybin Experience

Biomedicines February 18, 2020 Candace R. Lewis, Katrin H. Preller, B. Blair Braden et al. 27 citations

Psilocybin, the psychoactive compound in psilocybe mushrooms, primarily affects serotonin 2A receptors, which are highly expressed in the cingulate cortex. In healthy adults (n = 55) given oral psilocybin at low (0.160 mg/kg) or high (0.215 mg/kg) doses, greater thickness of the rostral anterior cingulate predicted higher subjective ratings on emotional sub-scales of the Five-Dimensional Altered State of Consciousness questionnaire, after controlling for sex and age. The caudal and posterior cingulate did not show this effect. These findings suggest that individual differences in brain structure, specifically cingulate cortex thickness, contribute to the wide variability in subjective psychedelic experiences, extending the traditional set and setting hypothesis.

Inhibition of Microglial GSK3β Activity Is Common to Different Kinds of Antidepressants: A Proposal for an In Vitro Screen to Detect Novel Antidepressant Principles.

Biomedicines March 7, 2023 Hans O Kalkman 16 citations

Depression involves an infection-like inflammation in the brain driven by activation of microglial Toll-like receptors and the enzyme glycogen synthase kinase-3β (GSK3β). GSK3β shifts the balance between the pro-inflammatory transcription factor NFκB and the neuroprotective, anti-inflammatory transcription factor NRF2. Tricyclic antidepressants work by activating GS-coupled microglial receptors, raising cAMP, and activating protein kinase A, which inhibits GSK3β. Other antidepressant principles—cannabinoid receptor-2 activation, opioid μ receptor agonists, 5HT2 agonists, valproate, ketamine, and vagus nerve stimulation—also inhibit GSK3β. Screening for NRF2 activation in microglial cells with TLR-activated GSK3β activity could accelerate discovery of novel antidepressants.

Age, Dose, and Locomotion: Decoding Vulnerability to Ketamine in C57BL/6J and BALB/c Mice.

Biomedicines June 25, 2023 Wen-Chien Chen, Tzong-Shi Wang, Fang-Yu Chang et al. 12 citations

Ketamine, a drug abused for its psychedelic effects, alters movement differently depending on a mouse's age, strain, and dose. Adolescent and adult male mice from two strains, C57BL/6J and BALB/c, were given ketamine at 0, 25, or 50 mg/kg after a 30-minute baseline. In C57BL/6J mice, both age groups showed significantly increased distance traveled and speed after ketamine, but BALB/c mice did not. The higher dose delayed the onset of hyperlocomotion compared to the lower dose. Adolescent C57BL/6J mice showed greater locomotor activation than adults, a difference not seen in BALB/c mice. These results indicate that sensitivity to ketamine's effects on movement is biologically determined.

Therapeutic Properties of Ayahuasca Components in Ischemia/Reperfusion Injury of the Eye

Biomedicines April 26, 2022 Anna Szilágyi, Barbara Takács, Réka Szekeres et al. 10 citations

A study tested whether N’N-dimethyltryptamine (DMT), a psychedelic compound, could protect the retina from damage caused by ischemia (restricted blood flow) followed by reperfusion. Because DMT is rapidly broken down by monoamine oxidase A (MAO-A), it was given alongside harmaline (a MAO-A inhibitor found in the Amazonian brew ayahuasca). In rats whose eye blood flow was blocked for 60 minutes and then restored for 7 days, harmaline alone protected the retina from injury. Surprisingly, adding DMT counteracted that protection. The two drugs had opposing effects on proteins involved in cell death, inflammation, tissue breakdown, and oxidative stress. The results suggest harmaline may have therapeutic potential for ischemic eye diseases, while DMT's effects on eye ischemia warrant caution.

Activation of σ1-Receptors by R-Ketamine May Enhance the Antidepressant Effect of S-Ketamine

Biomedicines September 28, 2023 Hans O. Kalkman 8 citations

Ketamine is a racemic mixture of S-ketamine and R-ketamine, each with distinct pharmacological activities. S-ketamine blocks NMDA channels and acts as an opioid μ-receptor agonist, while R-ketamine binds to σ1-receptors and is believed to act as an agonist. In patients with major depressive disorder, S-ketamine nasal spray shows clear antidepressant activity, but compared to intravenous racemic ketamine, its response rate, duration of action, and anti-suicidal activity appear less pronounced. The σ1-activity of R-ketamine may enhance pathways through which S-ketamine produces antidepressant effects and provide its own antidepressant activity, potentially explaining the superior effect of racemic ketamine over S-ketamine alone.

Gastrodin Mitigates Ketamine-Induced Inhibition of F-Actin Remodeling and Cell Migration by Regulating the Rho Signaling Pathway.

Biomedicines March 6, 2025 Ping-Cheng Shih, I-Shiang Tzeng, Yi-Chyan Chen et al. 1 citation

Gastrodin, a compound from the traditional herbal medicine Gastrodia elata, counteracts ketamine-induced disruptions in Rho signaling, cytoskeletal dynamics, and cell migration. In B35 and C6 cells, gastrodin reversed ketamine's effects on cell mobility inhibition, F-actin condensation, and the modulation of Rho pathway proteins including RhoGDI1, RhoA, CDC42, Rac1, ROCK1, NWASP, MLC2, PFN1, and cofilin-1. Similar modulations of Rho signaling were observed in the prefrontal cortex of Sprague Dawley rats. These findings suggest gastrodin may act as a comprehensive regulator of cellular signaling, with potential implications for neuronal function and cancer metastasis.

Psilocibin: Current Evidence, Safety Signals, and Challenges in Assessing Potential Multi-Organ Effects

Biomedicines July 6, 2026 Kasper Buczma, Katarzyna Kamińska, Kaja Kasarełło et al.

Psilocybin, a serotonergic hallucinogen, shows therapeutic promise for treatment-resistant depression, but its multi-organ safety profile remains unclear. This narrative review of preclinical studies, clinical data, and case reports found limited and heterogeneous evidence, with rare adverse events often confounded by polysubstance use and uncertain dosing. Biologically plausible mechanisms via serotonergic receptor activation warrant further investigation, yet controlled clinical data do not consistently indicate intrinsic multi-organ toxicity. The review highlights critical gaps in understanding psilocybin's organ-specific safety and calls for systematic evaluation.

Synaptic Plasticity-Intrinsic Excitability and Antidepressant Discovery.

Biomedicines June 1, 2026 Masaru Tanaka

Antidepressant efficacy, especially for rapid-acting agents like ketamine and esketamine, depends on coordinated phases of synaptic plasticity and neuronal excitability. Induction occurs through NMDAR and AMPAR signaling, consolidation involves TrkB, eEF2K, and SV2A to stabilize changes, and maintenance relies on intrinsic excitability governed by Kv7, HCN, and GIRK potassium channels that prevent relapse. The proposed Induction-Consolidation-Maintenance (ICM) framework links these phases to distinct therapeutic windows and candidate biomarkers such as SV2A PET, EEG, and fMRI, offering a hypothesis-generating roadmap for future studies rather than validated clinical tools.

Pharmacotherapeutic Options in Drug-Resistant Bipolar Depression: From Molecular Mechanisms to Rational Polypharmacotherapy.

Biomedicines May 23, 2026 Dominik Jucha, Michał Klimas, Dominika Wiśniewska et al.

About 25% of people with bipolar disorder experience drug-resistant depression, where standard treatments fail. This paper reviews augmentation strategies and polypharmacotherapy for treatment-resistant bipolar depression. Atypical antipsychotics show limited efficacy and high side effects. Ketamine produces the fastest and strongest antidepressant effect with low risk of switching to mania. Pramipexole shows long-term promise but carries high risk of mania and impulse control disorders. Celecoxib, as anti-inflammatory therapy, significantly improved response and remission rates compared to escitalopram alone. Memantine showed only early, short-term benefit. The authors conclude that rational polypharmacotherapy targeting glutamatergic and inflammatory pathways is most promising, with ketamine having the greatest clinical potential.

Ayahuasca and Its Main Component N,N-Dimethyltryptamine (DMT) for the Treatment of Mental Disorders: Mechanisms of Action, Clinical Studies, and Tools to Explore the Human Mind

Biomedicines February 25, 2026 Alice Melani, Giorgia Papini, Marco Bonaso et al.

Psychedelics are gaining renewed scientific interest as breakthrough therapies for mental disorders, with ayahuasca and its active component DMT showing particular promise. DMT acts primarily as a serotonin 5-HT2A receptor partial agonist, while 5-MeO-DMT has higher affinity for 5-HT1A receptors; both foster neuroplasticity and reorganize brain networks involved in perception, cognition, and mood. Current evidence offers an optimistic outlook for treatment-resistant depression and major depressive disorder, with four phase II studies of 5-MeO-DMT and one of DMT for TRD, plus two phase II studies of DMT fumarate for MDD. Evidence for other mental disorders remains preliminary.

Opioid Receptors in Psychedelia: Indirect Serotonergic Modulation of Direct KOR Activation by Salvinorin A

Biomedicines February 21, 2026 Maximiliano Ganado, Carmen Rubio, Javier Pérez-villavicencio et al.

Psychedelic effects are not solely driven by serotonin receptors. The compound salvinorin A, from Salvia divinorum, produces altered consciousness by directly activating kappa opioid receptors (KORs), bypassing serotonin entirely. This review synthesizes evidence from lab studies, animal models, and human brain imaging. Salvinorin A triggers a specific signaling pathway (β-arrestin bias) that causes rapid receptor desensitization, disrupts thalamocortical communication, suppresses dopamine activity in reward circuits, and fragments large-scale brain networks. Despite being a potent opioid agonist, it has low abuse potential because its aversive effects and dopamine suppression prevent positive reinforcement. Understanding opioid receptor mechanisms expands psychedelic neuroscience beyond serotonin-focused models and may guide development of treatments for depression, addiction, and chronic pain.