Forensic science international
December 17, 2003
Kenji Tsujikawa, Tatsuyuki Kanamori, Yuko Iwata et al.
79 citations
Hallucinogenic mushrooms circulating in Japan were analyzed using scanning electron microscopy and high-performance liquid chromatography. Psilocybe cubensis contained psilocin (0.14–0.42%) and psilocybin (0.37–1.30%) in the whole mushroom, with higher concentrations in the cap than the stem. Copelandia species contained psilocin (0.43–0.76%) and psilocybin (0.08–0.22%) in the whole mushroom, again with more alkaloid in the cap. Psilocybe cubensis is psilocybin-rich, while Copelandia is psilocin-rich. The combination of SEM and optical microscopy was effective for observing characteristic fungal tissues such as basidiomycetes, spores, cystidia, and basidia.
Journal of analytical toxicology
March 1, 2002
Tatsuyuki Kanamori, Hiroyuki Inoue, Yuko Iwata et al.
61 citations
In rats, the psychoactive compound 2C-B is broken down into at least six distinct breakdown products through two main metabolic pathways. One pathway converts 2C-B into an aldehyde, which is then further processed into an alcohol and a carboxylic acid. The other pathway produces metabolites where a methyl group is removed from either the 2- or 5-position of the molecule, followed by acetylation of the amino group. These findings indicate that the body metabolizes 2C-B through multiple chemical changes, resulting in a variety of metabolites that are excreted in urine.
JOURNAL OF HEALTH SCIENCE
April 1, 2003
T. Kanamori, K. Tsujikawa, Yoshihito Ohmae et al.
27 citations
After oral administration of 10 mg/kg of the psychoactive substance 2C-B to male Wistar rats, the main metabolite found in urine was 5-O-desmethyl-N-acetyl-2C-B, accounting for 13.2% of the dose. Other urinary metabolites included 2-O-desmethyl-N-acetyl-2C-B (5.8%), 2-O-desmethyl-2C-B (3.5%), a carboxylic acid compound (1.9%), 5-O-desmethyl-2C-B (1.2%), and an alcoholic compound (0.8%). Only 0.2% of the original 2C-B was excreted unchanged in urine. In faeces, only two acetylated metabolites were detected. Most hydroxyl-containing urinary metabolites were recovered as conjugates.